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Fig. 2 | Respiratory Research

Fig. 2

From: Urban particulate matter (PM) suppresses airway antibacterial defence

Fig. 2

PM exposure impairs the antimicrobial defence in airway epithelial cells to promote P. aeruginosa invasion. a BEAS-2B cells were incubated with 0, 10, 50, or 100 μg/ml of PM for 24 h, followed by infection with P. aeruginosa at various MOI of 1, 10, or 20 for an additional 2 h. CFU counts were performed to determine the number of viable P. aeruginosa. b and c BEAS-2B cells were incubated with 0, 10, 50, or 100 μg/ml of PM for 24 h, followed by infection with GFP- P. aeruginosa at an MOI of 10 for an additional 2 h. The mean MFI represents the intracellular GFP-P. aeruginosa. The results are expressed as the mean ± SD of three independent experiments. CFUs, colony-forming units; MFI, mean fluorescence intensity; MOI, multiplicities of infection. *P < 0.05 and **P < 0.01 versus P0 (0 μg/ml)

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