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Fig. 2 | Respiratory Research

Fig. 2

From: Upregulation of Mas-related G Protein coupled receptor X2 in asthmatic lung mast cells and its activation by the novel neuropeptide hemokinin-1

Fig. 2

Role of MRGPRX2 on HK-1-induced degranulation in LAD2 cells and transfected RBL-2H3 cells. a Expression of MRGPRX2 on LAD2 cells b Effects of NK-1R antagonist CP96345 (10 μM) on SP (1 μM) and HK-1 (3 μM)-induced degranulation in LAD2 cells. c MRGPRX2 expression on RBL-2H3 cells stably expressing the receptor (RBL-MRGPRX2). d Effects of CP96345 (10 μM) on SP (1 μM) and HK-1(3 μM)-induced degranulation in RBL-MRGPRX2 cells. e Transient expression of NK-1R in RBL-2H3 cells (RBL-NK-1R) and f Effects of CP93645 on SP (1 μM) and HK-1 (3 μM) on degranulation in RBL-NK-1R cells. g Western blotting to determine the expression of MRGPRX2 in control shRNA and MRGPRX2 shRNA-transduced LAD2 cells. β–actin was used as a loading control. h Effects of shRNA-mediated knockdown of MRGPRX2 on SP (0.3 μM) and HK-1 (3 μM)-induced degranulation in LAD2 cells. Flow cytometry (a, c and e) and Western blotting data (g) presented are representative of 3 similar experiments. Degranulation data (b, d, f and h) are the mean ± SEM of 3 independent experiments. Statistical significance was determined by two-way ANOVA with Bonferroni’s post test. *p < 0.01

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