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Fig. 1 | Respiratory Research

Fig. 1

From: Upregulation of Mas-related G Protein coupled receptor X2 in asthmatic lung mast cells and its activation by the novel neuropeptide hemokinin-1

Fig. 1

MRGPRX2 expression in human non-asthma and asthma lung. a Representative photomicrographs (n = 6) of immunofluorescence staining of human non-asthma and asthma lungs. Samples were stained with anti-human tryptase (green) and anti-MRGPRX2 antibody (red). Overlay of double stained samples are shown. Scale bar = 50 μm. b anti-tryptase stained slides were counted and data are presented as the number of MCs from 6 non-asthma and 6 asthma lungs. c MRGPRX2-positive MC number was counted in non-asthma vs asthma lung MCs and the data was represented as mean ± SEM of six lung samples. d MRGPRX2 intensity in 100 normal and asthma lung MCs was quantified using ImageJ software and data is represented as mean ± SEM. Statistical significance was determined by two-way ANOVA with Bonferroni’s post test

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