Fig. 4

Histological analysis of lung tissue section following IRB and the effect of Src kinase and ERK inhibition. a. Representative H&E lung tissue sections of ctr (upper left), 6 h IRB (upper right), 6 h IRB + Src Inh. (lower left) and 6 h IRB + ERK Inh. (lower right). Note that after 6 h IRB, lung histology reveals features of lung injury, such as focal membrane thickening, capillary congestion and inflammatory cell infiltration. b. Quantification of lung injury in lung tissue sections confirmed the increased total lung injury score after 6 h IRB, compared to control. Neither after Src inhibition nor after ERK inhibition, there was a significant change compared to 6 h IRB. Data presented as mean ± SEM, n = 6–10 per group, * p < 0.05 to ctr, white bar, control, black bar, 6 h IRB, dark grey bar, 6 h IRB + Src Inh, grey bar, 6 h IRB + ERK Inh