Fig. 1

Representative Western blot analysis of phosphorylated (p)-Src kinase at Tyr416 in lung tissue samples during IRB and the efficacy of the Src kinase inhibitor PP-2 (1 mg/kg ip) (representative western blot analysis, upper, band intensity quantification, lower). a. The ratio of p-Src to actin is elevated at 30 min of IRB, while returns to control levels after 3 and 6 h of IRB. Quantification of band intensities revealed a ~5× fold upregulation of p-Src to actin at 30 min of IRB (b). PP-2 administration inhibited Src phosphorylation during the entire IRB session. Data presented as mean ± SEM, n = 4–13 per group, *p < 0.05 to ctr, grey bars, 30 min IRB, dark grey bars, 3 h IRB, black bars, 6 h IRB