Table 1 Neutrophil elastase determination assays
Name | Samples | Type of measurement | Assay | Unit of measure | N° of test | Detection range | Limit of detection |
|---|---|---|---|---|---|---|---|
Human Elastase ELISA (Hycult Biotech) | Plasma, Cell culture supernatant | Active NE | ELISA | ng/mL | 2 × 96 | 0.4–25 ng/mL | 0.4 ng/mL |
ProteaseTag® Active NE Immunoassay (ProAxsis Ltd) | BAL, Sputum sol, Serum free media | Active NE | ELISA | ng/mL | 96 | 15.625–1000 ng/mL | 7.2 ng/mL |
Human PMN Elastase ELISA Kit (Abcam) | Cell culture supernatant, Serum, Plasma | PMN Elastase/alpha1-PI complex | ELISA | ng/mL | 96 | 0.16–10 ng/ml | 1.98 pg/mL |
Neutrophil elastase Human ELISA Kit, CE (eBioscience) | Cell culture supernatant, Serum, Plasma | PMN Elastase/alpha1-PI complex | ELISA | ng/mL | 96 | 0.16–10.0 ng/mL | 1.98 pg/mL |
Neutrophil Elastase Activity Assay Kit (Abcam) | Plasma, Whole Blood, Purified protein | Active NE | Fluorometric | ng | 100 | 1–25 ng | 1 ng |
Dynatech MR 5000 (Dynatech Corporation) | Sputum sol | Active NE | Spectrophotometry | mcgM | 96 | – | – |
Neutrophil Elastase Activity Assay Kit (Cayman Chemical) | Blood | Active NE | Fluorometric | mU/mL | 2 × 96 | 0–10 mU/mL | 0.156 mU/mL |