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Table 1 Neutrophil elastase determination assays

From: Neutrophil elastase in bronchiectasis

Name Samples Type of measurement Assay Unit of measure N° of test Detection range Limit of detection
Human Elastase ELISA (Hycult Biotech) Plasma, Cell culture supernatant Active NE ELISA ng/mL 2 × 96 0.4–25 ng/mL 0.4 ng/mL
ProteaseTag® Active NE Immunoassay (ProAxsis Ltd) BAL, Sputum sol, Serum free media Active NE ELISA ng/mL 96 15.625–1000 ng/mL 7.2 ng/mL
Human PMN Elastase ELISA Kit (Abcam) Cell culture supernatant, Serum, Plasma PMN Elastase/alpha1-PI complex ELISA ng/mL 96 0.16–10 ng/ml 1.98 pg/mL
Neutrophil elastase Human ELISA Kit, CE (eBioscience) Cell culture supernatant, Serum, Plasma PMN Elastase/alpha1-PI complex ELISA ng/mL 96 0.16–10.0 ng/mL 1.98 pg/mL
Neutrophil Elastase Activity Assay Kit (Abcam) Plasma, Whole Blood, Purified protein Active NE Fluorometric ng 100 1–25 ng 1 ng
Dynatech MR 5000 (Dynatech Corporation) Sputum sol Active NE Spectrophotometry mcgM 96
Neutrophil Elastase Activity Assay Kit (Cayman Chemical) Blood Active NE Fluorometric mU/mL 2 × 96 0–10 mU/mL 0.156 mU/mL
  1. Definitions: NE neutrophil elastase, alpha1-PI alpha-1 proteinase inhibitor; PMN polymorphonuclear leukocytes