Fig. 2
From: Reduced suppressive effect of β2-adrenoceptor agonist on fibrocyte function in severe asthma
Effect of cAMP modulation on fibrocyte number and differentiation. NANT cells from healthy subjects (n = 4) and severe asthma patients (n = 4) were treated with 8-Br-cAMP (10−4- 10−3 M for 3 days (a-b). Alternatively, NANT cells from severe asthma patients were treated with rolipram (10−5 M) in the presence or absence of salmeterol (10−9 M) for 3 days (c-d). The number of fibrocytes (Col I+/CD45+ cells; a and c) and differentiating fibrocytes (α-SMA+ cells; b and d) were determined. Data points represent mean ± SEM. * p < 0.05 versus vehicle-treated cells for each group