Fig. 1

Effect of salmeterol on the number, differentiation and CCR7 expression of fibrocytes. NANT cells from healthy subjects (n = 8–9) and patients with non-severe (n = 7) or severe asthma (n = 7–9) were treated with salmeterol (10⁻⁹- 10⁻⁷ M) for 3 days. Fibrocyte (Col I⁺/CD45⁺ cells; a) and differentiating fibrocyte (α-SMA⁺ cells; b) number, and percentage (c) and median fluorescence intensity (MFI; d) of CCR7⁺ fibrocytes (Col I⁺/CD45⁺/CCR7⁺ cells) were determined. Data points represent mean ± SEM. * p < 0.05 and ** p < 0.01 versus vehicle-treated cells for each group. ^# p < 0.05 healthy versus severe asthma and ^& p < 0.05 non-severe asthma versus severe asthma