Table 1 Genes upregulated by >1.5-fold in both HLF lines (L20 and L21) at 24 h, by degranulated products from eosinophils pre-activated with IL-3 for 20 h and seeded on coated HA-IgG (IL3IgG) for 6 h, from two different eosinophil donors, compared to HLF controls cultured with medium only, and with rhIL-3 (1 ng/ml) plus soluble HA-IgG (1 μg/ml)

Secreted Proteins

Apolipoprotein A-I (APOA1); bone morphogenetic protein 8b (BMP8B); C-C motif chemokine ligand 2 (CCL2); chemokine (C-C motif) ligand 4-like 2 (CCL4L2); chondroadherin like (CHADL); collagen type IV alpha 3 (COL4A3); complement component 1, q subcomponent-like 1 (C1QL1); complement component 3 (C3); complement component 8 alpha subunit (C8A); C-X-C motif chemokine ligand 1 (CXCL1); C-X-C motif chemokine ligand 8 (CXCL8); fibroblast growth factor 10 (FGF10); fibronectin leucine rich transmembrane protein 1 (FLRT1); gremlin 2, DAN family BMP antagonist (GREM2); interleukin 32 (IL32); interleukin 6 (IL6); Klotho (KL); mannosyl (alpha-1,3-)-glycoprotein beta-1,4-Nacetylglucosaminyltransferase, isozyme A (MGAT4A); nodal growth differentiation factor (NODAL); platelet factor 4 variant 1 (PF4V1); plexin domain containing 1 (PLXDC1); secreted and transmembrane 1 (SECTM1); SPARC like 1 (SPARCL1); urocortin 2 (UCN2)

Membrane proteins

ADAM metallopeptidase domain 20 (ADAM20); ATPase phospholipid transporting 8B4 (ATP8B4); bone marrow stromal cell antigen 2 (BST2); carnitine palmitoyltransferase 1B (CPT1B); CD177 molecule (CD177); cholesterol 25-hydroxylase (CH25H); complement component 8 alpha subunit (C8A); chromosome 1 open reading frame 204 (C1orf204); chromosome 2 open reading frame 82 (C2orf82); cytochrome P450 family 1 subfamily A member 1 (CYP1A1); cytochrome P450 family 1 subfamily B member 1 (CYP1B1); dachsous cadherin-related 2 (DCHS2); fibronectin leucine rich transmembrane protein 1 (FLRT1); G protein-coupled receptor 17 (GPR17); galactose-3-O-sulfotransferase 2 (GAL3ST2); glutamyl aminopeptidase (ENPEP); guanine nucleotide binding protein (G protein), gamma 3 (GNG3); immunoglobulin superfamily member 6 (IGSF6); intercellular adhesion molecule 1 (ICAM1); interleukin 12 receptor subunit beta 1 (IL12RB1); Janus kinase 3 (JAK3); Klotho (KL); mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme A (MGAT4A); NPC1-like 1 (NPC1L1); NTPase, KAP family P-loop domain containing 1 (NKPD1); nucleoporin 210 kDa (NUP210); PAPPA antisense RNA 1 (PAPPA-AS1); par-6 family cell polarity regulator beta (PARD6B); plexin domain containing 1 (PLXDC1); potassium voltage-gated channel modifier subfamily S member 2 (KCNS2); protein tyrosine phosphatase, receptor type N2 (PTPRN2); RAB9B, member RAS oncogene family (RAB9B); receptor (chemosensory) transporter protein 4 (RTP4); reticulon 1 (RTN1); secreted and transmembrane 1 (SECTM1); shroom family member 2 (SHROOM2); solute carrier family 1 member 3 (SLC1A3); solute carrier family 16 member 6 (SLC16A6); solute carrier family 18 member A2 (SLC18A2); solute carrier family 22 member 14 (SLC22A14); solute carrier family 51 alpha subunit (SLC51A); sortilin related VPS10 domain containing receptor 2 (SORCS2); synapse differentiation inducing 1 (SYNDIG1); TBC1 domain family member 3H (TBC1D3H); TMEM110-MUSTN1 readthrough (TMEM110-MUSTN1); V-set and immunoglobulin domain containing 10 like (VSIG10L); zinc finger CCCH-type containing 12A (ZC3H12A)

Nuclear proteins

Apolipoprotein A-I (APOA1); brain expressed X-linked 2 (BEX2); calmodulin like 6 (CALML6); cell death inducing DFFA like effector c (CIDEC); cilia and flagella associated protein 46 (CFAP46); distal-less homeobox 1 (DLX1); ETS homologous factor (EHF); family with sequence similarity 71 member E1 (FAM71E1); fibroblast growth factor 10 (FGF10); forkhead box D2 (FOXD2); hes family bHLH transcription factor 1 (HES1); histone cluster 1, H2bi (HIST1H2BI); histone cluster 2, H2be (HIST2H2BE); histone cluster 2, H3d (HIST2H3D); inhibitor of CDK, cyclin A1 interacting protein 1 (INCA1); LIM homeobox 4 (LHX4); NFKB inhibitor alpha (NFKBIA); NFKB inhibitor zeta (NFKBIZ); par-6 family cell polarity regulator beta (PARD6B); phosphatase domain containing, paladin 1 (PALD1); SRY-box 7 (SOX7); ubiquitin specific peptidase 17-like family member 1 (USP17L1); vitamin D (1,25-dihydroxyvitamin D3) receptor (VDR); v-rel avian reticuloendotheliosis viral oncogene homolog B (RELB); zinc finger CCCH-type containing 12A (ZC3H12A); zinc finger MYND-type containing 15 (ZMYND15); ZNF559-ZNF177 readthrough (ZNF559-ZNF177)