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Fig. 1 | Respiratory Research

Fig. 1

From: RNA-sequencing analysis of lung primary fibroblast response to eosinophil-degranulation products predicts downstream effects on inflammation, tissue remodeling and lipid metabolism

Fig. 1

Evaluation of eosinophil degranulation by the measurement of EDN in eosinophil conditioned media. To prepare the eosinophil conditioned media that will be tested on human lung fibroblasts, human blood eosinophils were activated with IL-3 or IL-5 for 20 h and were then added on heat-aggregated (HA) human serum IgG for 6 h. Three conditioned media were prepared, including two conditioned media from IL-3-activated eosinophils, cultured for 6 h on coated HA-IgG (IL3IgG) or uncoated wells (IL3). The third conditioned medium was from IL-5-activated eosinophils seeded for 6 h on HA-IgG (IL5IgG). EDN released from eosinophils in the three conditioned media was measured by ELISA. The graph is an average ± SEM of three different cultures from three different eosinophil donors. The three groups were statistically different from each other as determine using the One-Way Analysis of Variance followed by the Holm-Sidak method (p < 0.001, n = 3)

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