Fig. 5

Blocking lipid rafts aggregation prevented Ca²⁺ influx induced by IL-4. Intracellular Ca²⁺ levels were recorded using Fura-2/AM. BECs were pretreated with or without 10 mM M-βCD. Then, the fluorescence of Fura-2 was detected in cells with a stimulation of 20 ng/ml IL-4. Ca²⁺ signals were recorded for calculating the fluorescence intensity ratio of F340/F380. (a-d) Representative trace of calcium responses. (e and f) Changes in Ca²⁺ influx duration and amplitude. Results are expressed as mean ± SD; n = 12. *P < 0.05 versus BSA, ^# P < 0.05 versus IL-4 treated group