Fig. 3

Nintedanib inhibits the phosphorylation of growth factor receptors on fibroblasts induced by fibrocytes. Human lung fibroblasts (MRC-5) were incubated with nintedanib at different concentrations and with the cell culture supernatant of fibrocytes. The expression of all receptors and receptor phosphorylation were measured by an immunoblot analysis. Representative immunoblot (a) and corresponding densitometric quantification of PDGFR phosphorylation (b; n = 3 independent experiments) and FGFR phosphorylation (c; n = 3 independent experiments). Data were analyzed using a one-way ANOVA and are displayed as means ± SDs in three separate experiments. For all graphs: *P < 0.05 versus the value in the group treated with fibrocyte supernatants alone