Fig. 4

Induction of lung epithelial and endothelial cell damage by ARDS patient’s BALF. It showed that ARDS patients’ BALF could drastically induce human lung epithelial and endothelial cell damage after overnight incubation, whereas addition of anti-histone H4 antibody or heparin remarkably inhibited cell death caused by ARDS patient’s BALF. Likewise, LDH levels were significantly increased in supernatant of these cells after overnight incubation of ARDS patient’s BALF, whereas addition of anti-histone H4 antibody or heparin could reduce LDH levels. Variables were expressed as mean ± standard deviation (SD)