Fig. 6

Antiviral and cytokine mRNA induction in the lung was restored in RIG-I TG mice during influenza infection. For CS groups, RIG-I WT and TG mice were exposed to CS for 6 weeks in a smoke exposure chamber. Mice were intranasally infected with a non-lethal dose (300 pfu/mouse) of the IAV PR8 strain. Mock treated mice were inoculated with PBS. At the indicated time points, the mice were sacrificed, and lung tissues were collected for RNA extraction. IFN and cytokine mRNA levels were assessed by qRT-PCR and normalized to β-actin. Data are expressed as means ± SEM (n = 9). * denotes a significant difference compared to the WT CS PR8 group (P < 0.05)