Fig. 1

RIG-I and IAV NP expression in RIG-I TG and WT mice. I IAV-infected RIG-I TG and WT mice express RIG-I in isolated type II alveolar epithelial cells (AEC II). AEC II were infected with IAV PR8 at an MOI of 6 and incubated for an additional 24 h to stimulate RIG-I production. The cells were processed for immunohistochemistry (A-L) for detection of RIG-I (red, B, E, H and K) and surfactant protein C (SPC, green, C, F, I and L). Nuclei were stained with DAPI (blue) and were shown in overlaid images with RIG-I and SPC (A, D, J and I). Scale bars = 50 μm. II Mice were first intranasally infected with IAV PR8 (300 pfu/mouse) or mock infected with PBS. Mouse lungs were processed for immunohistochemistry for detection of RIG-I protein (green) or IAV NP (red). The lungs of 3 mice from each treatment group were processed for immunohistochemistry, and results shown were typical for the groups. The bar represents 100 μm for all the images