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Table 2 Summary of AT2 cell isolation methods

From: Fraction of MHCII and EpCAM expression characterizes distal lung epithelial cells for alveolar type 2 cell isolation

Author

Year

Preparation of single cells

Cell selection

Surface antigens for cell isolation

Cell sorting modality

Purity assessment

Purity

Ref.

Corti et al.

1996

Dispase and LMP agarose

N

CD16/32, CD45

Magnet

PAP staining

92.8%

13

Rice et al.

2002

Dispase and LMP agarose

N

CD45, CD16/32

Antibody-coated plate

PAP staining, SP-C (ICC)

>90%

14

Kim et al.

2005

Dispase and LMP agarose

N

CD31, CD45, Sca-1

FACS

SP-C (IF)

N/A

26

Herold et al.

2006

Dispase and LMP agarose

N

CD16/32, CD45

Magnet

Papanicolaou staining, proSP-C (IF)

>90%

27

Bernice et al.

2008

Dispase and LMP agarose

N

CD45, CD11b, CD11c

Magnet

Papanicolaou staining

71%

15

Marsh et al.

2009

Dispase and LMP agarose

N

CD16/32, CD45

Magnet

proSP-C (FACS)

>80%

28

Teisanu et al.

2011

Elastase

N and P

CD45, CD31, EpCAM, Sca-1

FACS

intrinsic GFP (FACS)

N/A

18

Messier et al.

2012

Dispase and LMP agarose

N and P

CD45, EpCAM

Magnet

SP-A (FACS)

91.1

29

Yamada et al.

2013

Dispase II and collagenase

N and P

CD45, VE-cadherin, EpCAM

FACS

N/A

N/A

19

Yamada et al.

2013

Dispase and LMP agarose

N and P

CD45, EpCAM

Magnet

proSP-C (IF and FACS)

>96%

19

Lee et al.

2013

Dispase and LMP agarose

N and P

CD45, CD31, CD74

FACS

intrinsic GFP (FACS)

91.8%

17

Hasegawa et al.

2015

Dispase and LMP agarose

N and P

CD45, CD31, EpCAM, MHCII

FACS

proSP-C (IF and FACS)

98–99%

 
  1. LMP agarose low melting point agarose, N negative selection, P positive selection, EpCAM epithelial cell adhesion molecule, VE-cadherin vascular endothelial cadherin, Magnet magnet-based cell isolation, FACS fluorescence-activated cell sorting, SP-C surfactant protein-C, SP-A surfactant protein-A, ICC immunocytochemistry, IF immunofluorescence