Traditional | New method | |
---|---|---|
Material | Peripheral blood | Peripheral blood |
Volume needed | 10 mL | 100 μL |
Processing | Isolate PBMC by density centrifugation (Ficoll-Paque) ⇩ Extracellular antibodies added ⇩ Cell fixation and permabilization ⇩ Intracellular antibodies added | Add antibodies and incubate. ⇩ Red blood cell lysis solution added |
Cell washing steps | 8 | 0 |
Detection method | Flow cytometry | Flow cytometry |
Fibrocyte Concentration | Estimate percentage of total PBMC or per mL of blood | Absolute concentration using beads |
Processing time | Over 3 h | Under 1 h |
Advantages | Unused isolated PBMC can be used for other purposes (e.g. culture or stored) | Fast and small blood volume Reduced cell loss due to no washing steps or permabilization |
Reference | [22] | Method section or [13] |