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Table 3 Comparison of methods to measure circulating fibrocytes in peripheral blood

From: Fibrocyte measurement in peripheral blood correlates with number of cultured mature fibrocytes in vitro and is a potential biomarker for interstitial lung disease in Rheumatoid Arthritis

 

Traditional

New method

Material

Peripheral blood

Peripheral blood

Volume needed

10 mL

100 μL

Processing

Isolate PBMC by density

centrifugation (Ficoll-Paque)

Extracellular antibodies added

Cell fixation and permabilization

Intracellular antibodies added

Add antibodies and incubate.

Red blood cell lysis solution added

Cell washing steps

8

0

Detection method

Flow cytometry

Flow cytometry

Fibrocyte Concentration

Estimate percentage of total PBMC or per mL of blood

Absolute concentration using beads

Processing time

Over 3 h

Under 1 h

Advantages

Unused isolated PBMC can be used for other purposes (e.g. culture or stored)

Fast and small blood volume

Reduced cell loss due to no washing steps or permabilization

Reference

[22]

Method section or [13]