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Fig. 5 | Respiratory Research

Fig. 5

From: Stromal derived factor-1 mediates the lung regenerative effects of mesenchymal stem cells in a rodent model of bronchopulmonary dysplasia

Fig. 5

Effect of SDF-1 Knockdown on MSC Anti-inflammatory Effects in Experimental BPD. a Lung sections stained with MAC-3 (brown) showing increased MAC-3 positive cells/HPF in hyperoxic animals treated with IT MSC-SDF KD. Original magnification X 200. Scale bars are 100 μm. b IT MSC-NS control significantly reduced MAC-3 positive cells/HPF and (c) broncho-alveolar lavage fluid macrophage count in hyperoxic animals. SDF-1 knockdown decreased this beneficial effect of MSC. d No differences in broncho-alveolar lavage fluid neutrophil count between the hyperoxic MSC treated groups. e Similar decrease in lung IL-1β expression in hyperoxia MSC-NS control and MSC-SDF KD groups. A representative Western Blot normalized to β-Actin is showed in the lower panel. f Increased lung Il-10 concentration in both hyperoxic MSC treated groups but this is blunted in the hyperoxia MSC-SDF KD group. (*P < 0.05; normoxia vs. hyperoxia-placebo, ** P < 0.05; hyperoxia-placebo vs hyperoxia MSC-NS control or hyperoxia MSC-SDF KD, † P < 0.05; hyperoxia MSC-NS control vs. hyperoxia MSC-SDF KD; N = 5/group). White bars are RA and black bars are hyperoxia. HYP is hyperoxia

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