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Table 1 The effect of LPS on cytokine levels in the supernatants of MDM and LM cultures

From: Human lung and monocyte-derived macrophages differ with regard to the effects of β2-adrenoceptor agonists on cytokine release

  Monocyte-derived macrophages Lung macrophages
Cytokine LPS - LPS + LPS - LPS +
TNF-α 1.8 ± 0.5 16.4 ± 6.5 0.7 ± 0.1 33.8 ± 7.1
IL-1ß 0.05 ± 0.01 0.09 ± 0.02 0.07 ± 0.03 0.54 ± 0.18*
IL-6 1.3 ± 0.5 13.2 ± 3.6 0.2 ± 0.1* 229.2 ± 80.3*
CCL2 2.2 ± 0.6 9.7 ± 2.4 3.1 ± 0.9 19.6 ± 3.9*
CCL3 4.5 ± 2.5 22.2 ± 8.1 3.1 ± 0.8 148.7 ± 41.2*
CCL4 6.5 ± 2.3 86.5 ± 31.4 6.8 ± 2.3 264.2 ± 78.6
IL-8 5.5 ± 2.3 93.2 ± 32.1 39.3 ± 14.3 1411.4 ± 383.1*
  1. Macrophages were incubated with medium only (LPS-) or 10 ng.ml−1 LPS in medium (LPS+) for 24 h. Cell culture supernatants were collected and analyzed using an ELISA. Data are expressed as the mean ± SEM ng.10−6 cells from 5 to 8 experiments. Asterisks indicate significant differences (Wilcoxon’s t test) between MDM and LM experiments