Human lung and monocyte-derived macrophages differ with regard to the effects of β2-adrenoceptor agonists on cytokine release

Table 1 The effect of LPS on cytokine levels in the supernatants of MDM and LM cultures

	Monocyte-derived macrophages		Lung macrophages
Cytokine	LPS -	LPS +	LPS -	LPS +
TNF-α	1.8 ± 0.5	16.4 ± 6.5	0.7 ± 0.1	33.8 ± 7.1
IL-1ß	0.05 ± 0.01	0.09 ± 0.02	0.07 ± 0.03	0.54 ± 0.18*
IL-6	1.3 ± 0.5	13.2 ± 3.6	0.2 ± 0.1*	229.2 ± 80.3*
CCL2	2.2 ± 0.6	9.7 ± 2.4	3.1 ± 0.9	19.6 ± 3.9*
CCL3	4.5 ± 2.5	22.2 ± 8.1	3.1 ± 0.8	148.7 ± 41.2*
CCL4	6.5 ± 2.3	86.5 ± 31.4	6.8 ± 2.3	264.2 ± 78.6
IL-8	5.5 ± 2.3	93.2 ± 32.1	39.3 ± 14.3	1411.4 ± 383.1*

Macrophages were incubated with medium only (LPS-) or 10 ng.ml⁻¹ LPS in medium (LPS+) for 24 h. Cell culture supernatants were collected and analyzed using an ELISA. Data are expressed as the mean ± SEM ng.10⁻⁶ cells from 5 to 8 experiments. Asterisks indicate significant differences (Wilcoxon’s t test) between MDM and LM experiments

ISSN: 1465-993X