Fig. 3
Effects of formoterol, PGE2 and roflumilast on LPS-induced TNF-α, CCL2, CCL3 and CCL4 release from LMs. Cells were pre-incubated with indomethacin (1 μM) for 30 min, followed by incubation with roflumilast (1 nM or 100 nM), PGE2 (10 nM), formoterol (10 nM) or vehicle for another 30 min prior to stimulation with LPS (10 ng.ml−1) for 24 h). The data represent the mean ± SEM of 6 different experiments, *p < 0.05, **p < 0.01, ***p < 0.001 vs. LPS + PGE2 treatment; #p < 0.05 vs. LPS