Fig. 1

Azithromycin Decreases NALP3 Protein in THP-1 Monocytes. a THP-1 cells were treated with azithromycin at concentrations shown for 12 h overnight prior to immunoblot analysis for indicated proteins. b THP-1 cells were plated and rested overnight. Cells were treated with LPS at concentrations shown for 4 h prior to immunoblot analysis. c THP-1 cells were treated with azithromycin at concentrations shown for 12 h overnight and then treated with LPS for 4 h prior to immunoblot analysis. The data representative of 2–3 separate experiments. d Peripheral blood mononuclear cells were treated with azithromycin or clarithromycin at concentrations shown for 12 h overnight. Plate-adherent monocytes were then treated with LPS 500 ng/ml for 4 h prior to lysis and immunoblot analysis for indicated proteins. Data shown is representative of multiple experiments. e NALP3:actin densitometry of immunoblot shown in Figure D