Fig. 1

IPF fibroblast derived supernatants affect normal fibroblast migration and detachment in a fibronectin dependent manner. IPF/normal supernatants were added to fibroblast cells seeded on plastic or fibronectin (FN) (10 μg/ml) coated plates for 30 min (a-b) and 24 h (e-f). Following culture, cells were harvested for protein/RNA extraction and the effect of IPF derived supernatants (IPF-SN) on PCNA (a) and caspase-3 (b) levels were tested by western blotting (n = 4). The effect of the IPF-SN on fibroblast cell migration was tested by scratch test (c). Following the scratch test, cell detachment was quantified by crystal violet staining (d) (n = 9 and n = 4 for plastic and FN, respectively). Collagen 1a (COL1A) (e) and αSMA (ACTA2) (f) mRNA levels were tested by qPCR (n = 4). Results were normalized to control and considered significant * if p < 0.05, error bar represents the standard error