Fig. 6
a Representative Western blot of phosphorylated p-eNOS in HPMEC (n = 3). VEGF165a significantly increased phosphorylation of eNOS at 5 and 10 min in contrast with VEGF165b stimulation which did not induce any changes. b & c Measurement of HPMEC resistance by Endohm system. Dotted lines correspond to cell pre-incubated with inhibitor. L-NAME inhibited the effect of VEGF165a (b) and the effect of VEGF165b (c). d Graphs represent the mean of the scratched area for HPMEC treated with L-NAME inhibitor at 24 h relative to control. VEGF165a but not VEGF165b stimulation significantly diminished scratched area (p = 0.009) and was inhibited by L-NAME (p = 0.04). Densitometry and scratch assay were analysed by Kruskal-Wallis test with post hoc Dunn’s analysis. TEER measurement data were analysed using two-way ANOVA and Bonferroni post-test for multiple analysis. All data were plotted as mean ± SEM (n = 3-6)