Fig. 1
HPMEC stimulated with 20 ng/ml of VEGF165a, VEGF165b or without any stimulation (control). a Paracellular permeability was quantified by TEER using endohm-12 system in HPMEC cultured in inserts. VEGF165a reduced resistance (increased permeability) *p < 0.05 (45 min onwards) and VEGF165b increased resistance (decreased permeability) ***p < 0.001 (15 to 45 min) compared to control. b ECIS measurements on HPMEC show that VEGF165a reduced the resistance (increased permeability) significantly***p < 0.001 in comparison to control; whereas VEGF165b had an opposite effect with a significant increase (*p < 0.05) in the resistance (reduced permeability). Data expressed as a mean fold-change compared to control over time. c The passage of FITC-coupled BSA across the monolayer of HPMEC was monitored over a period of 90 min. The fluorescence intensity of the aliquots was quantified and data was expressed as cumulative FITC-BSA over time. Concentration of FITC-BSA in the lower chamber slowly increased in both control cells and those stimulated with VEGF165b; in contrast cells stimulated with VEGF165a showed a significant increase in the passage of FITC-BSA p < 0.01 after 60 min. Each experiment was performed in triplicate (n = 5-8). All data were analysed using two-way ANOVA and Bonferroni post-test for multiple analysis and plotted as mean ± SEM