Fig. 5

PFD attenuates bleomycin (BLM)-induced lung fibrosis development in PARK2 KO mice. a Photomicrographs of Hematoxylin & Eosin (H-E) and Masson trichrome staining of mouse lungs at day 21. Original magnification × 200. The right panel shows the average (±SEM) percentage of positively stained areas in Masson trichrome staining quantified using Image J. Treatment groups were composed of vehicle treated wild type (n = 10), BLM-treated wild type (n = 10), BLM-treated wild type with subsequent PFD (n = 10), BLM-treated PARK2 KO (n = 6), and BLM-treated PARK2 KO with subsequent PFD (n = 6). *p < 0.05. N.S.: not statistically significant. b Shown in the panel is the average (±SEM) soluble collagen measurement from Sircol assay using vehicle treated wild type (n = 10), BLM-treated wild type (n = 10), BLM-treated wild type with subsequent PFD (n = 10), BLM-treated PARK2 KO (n = 6), and BLM-treated PARK2 KO with subsequent PFD (n = 6) at day 21. *p < 0.05. N.S.: not statistically significant. c Immunohistochemical staining of 8-hydroxy-2-deoxyguanosine (8-OHdG), oxidized derivative of deoxyguanosine. Original magnification × 200 (d) Immunohistochemical staining of 4-hydroxy-2-nonenal (4-HNE) of lipid peroxidation. Original magnification × 200 (E) Cell counts in broncho-alveolar lavage fluid (BALF). BALF collection was performed at day 21. Cells were counted using a hemocytometer. Differential cell counts in BALF were analyzed from 300 cells stained with Diff-Quick. *p < 0.05, **p < 0.001. N.S.: not statistically significant