Fig. 3

PFD attenuates myofibroblast differentiation during insufficient autopagy/mitophagy in LF. a WB using anti-type I collagen, anti-α-SMA, and anti-β-actin of cell lysates from control siRNA (lane 1 ~ 4), ATG5 siRNA (lane 5 ~ 8) transfected LF. TGF-β (2 ng/ml) and PFD (500 μg/ml) treatment was started 48 h post transfection and protein samples were collected after 24 h treatment. In the right panels are the average (±SEM) taken from five independent experiments shown as relative expression. *p < 0.05. b WB using anti-type I collagen, anti-α-SMA, and anti-β-actin of cell lysates from control siRNA (lane 1 ~ 4), ATG7 siRNA (lane 5 ~ 8) transfected LF. TGF-β (2 ng/ml) and PFD (500 μg/ml) treatment was started 48 h post transfection and protein samples were collected after 48 h treatment. In the right panels are the average (±SEM) taken from five independent experiments shown as relative expression. *p < 0.05. c WB using anti-type I collagen, anti-α-SMA, and anti-β-actin of cell lysates from control siRNA (lane 1 ~ 4), PARK2 siRNA (lane 5 ~ 8) transfected LF. TGF-β (2 ng/ml) and PFD (500 μg/ml) treatment was started 48 h post transfection and protein samples were collected after 24 h treatment. In the right panels are the average (±SEM) taken from five independent experiments shown as relative expression. *p < 0.05