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Fig. 9 | Respiratory Research

Fig. 9

From: Human adipose-derived mesenchymal stem cells alleviate obliterative bronchiolitis in a murine model via IDO

Fig. 9

Effect of Treg depletion on IDO expression in tracheal allografts. C57BL/6 mice were transplanted heterotopically with tracheas from BALB/c. Human ASCs (1 × 106/0.2 ml) or PBS were administered to the recipient mice via tail veins on days 0, 1, 3, and 5 post-transplant. Also, mice were treated with or without 150 mg/Kg cyclophosphamide intraperitoneally on days 0, 1, 3, and 5 post-transplant to deplete Treg. a Percentages of splenic CD4+CD25+Foxp3+ Treg on day 7 were shown in mice with and without Treg depletion. b Tracheal grafts were harvested on day 7 post-transplant. IDO expression in the grafts were determined via quantitative RT-PCR. β-actin mRNA was served as internal control. All data are expressed as mean ± SEM; n = 5 per group. *, p < 0.05

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