Fig. 8

IDO inhibitor on the frequency of Treg and IL-10+/TGF-β+ Treg in recipient spleens. C57BL/6 mice were transplanted heterotopically with tracheas from BALB/c or C57BL/6 mice. Spleens were harvested on day 14 post-transplant. a Splenic cells were gated with CD4 and analyzed for CD25+ and Foxp3+ phenotype expression using flow cytometry. Representative dot plots were shown for the four experimental groups. b Frequency of splenic CD4+CD25+Foxp3+ Treg in each treatment group was summarized graphically. c Representative flow cytometry plots of the IL-10+ and TGF-β+ Treg in recipient spleens were presented. Spleen cells were initially gated by CD4 followed by CD25 and Foxp3. The CD4+CD25+Foxp3+ Treg were further gated for IL-10 and TGF-β. d The bar graphs summarized the percentages of IL-10+ and TGF-β+ Treg in isograft, allograft, allograft + ASCs, and allograft + ASCs + 1-MT. All data are expressed as mean ± SEM; n = 5 per group. *, p < 0.05 and **, p < 0.01