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Fig. 5 | Respiratory Research

Fig. 5

From: Extracellular heat shock protein 90α mediates HDM-induced bronchial epithelial barrier dysfunction by activating RhoA/MLC signaling

Fig. 5

HDM and hrHsp90α increased the levels of RhoA activity and MLC phosphorylation. a and b 16HBE cells were treated with HDM (200, 400, 600, 800, or 1200 U/ml) for 24 h or stimulated with HDM (400 U/ml) for 3, 6, 12, 24, or 48 h. MLC and p-MLC were detected. c Cells were exposed to HDM (400 U/ml), hrHsp90α (10, 40 μg/ml) or hrHsp90β (10, 40 μg/ml) for 24 h. MLC and p-MLC were detected. d Cells exposed to HDM (400 U/ml) for 1, 3, 6, 12, 24 h. Then RhoA activity was measured by Rho G-LISA RhoA activation assay kitTM biochem kit. e 16HBE cells pretreated with 1G6-D7 (30 μg/ml) for 2 h and then stimulated with HDM (400 U/ml), hrHsp90α (10 μg/ml) or hrHsp90β (10 μg/ml) for 6 h. RhoA activity was measured. Data are mean ± SD of three independent experiments.* P < 0.05 versus con group; # P < 0.05 versus HDM group

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