Fig. 2
From: Protein antigen of bird-related hypersensitivity pneumonitis in pigeon serum and dropping
Proteins from pigeon serum (a) and pigeon dropping extract (PDE) (b) were separated by 2-DE and visualized by SYPRO Ruby staining. Representative results of 2-DE immunoblotting of pigeon serum (c) and PDE (d) using sera from BRHP patients are shown. The 1-DE immunoblot of each sample is shown in the right panel for comparison. Proteins in the 26 kDa band observed on 1-DE immunoblotting (indicated by arrow) were separated into several protein spots (indicated within broken-lined boxes). Sera from BRHP patients reacted with 4 spots from the proteins from pigeon serum and 5 spots from the PDE in a similar pH range. M, Molecular weight markers; 1-DE IB, 1-DE immunoblot