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Fig. 2 | Respiratory Research

Fig. 2

From: Exogenous gene transfer of Rab38 small GTPase ameliorates aberrant lung surfactant homeostasis in Ruby rats

Fig. 2

Expression of Rab38 protein in LEC type II cells infected with Ad-Rab38. (a) Western blot shows expression of Rab38 protein by Ad-Rab38 infection from day 1 through day 21. (b) The β-galactosidase (lacZ) assay shows ~100% of transduction efficiency of rat type II cells by Ad-lacZ infection at MOI = 5. Bar = 20 μm. (c) Immunofluorescence staining of exogenously expressed Rab38 in LEC type II cells. Adherent cells were infected with Ad-lacZ (a – d) or Ad-Rab38 (e – l). Objective lens magnification, a − h: ×40, i − l:×100. Bar = 25 μm. The exogenous Rab38 appeared to at least partially co-localize with granularly distributed SP-B in the cells (l, arrow heads)

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