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Fig. 6 | Respiratory Research

Fig. 6

From: Ku80 correlates with neoadjuvant chemotherapy resistance in human lung adenocarcinoma, but reduces cisplatin/pemetrexed-induced apoptosis in A549 cells

Fig. 6

Ku80 overexpression caused the resistance to apoptosis induced by cisplatin/pemetrexed in A549 cells. a Flow cytometry apoptosis detection. A549 cells were transfected with shRNA and full length of cDNA to up- and down-regulate Ku80 expression, respectively. Cells were treated with 0.9 μM mixed drugs (cisplatin combined with pemetrexed) for 24 h. b Apoptosis rate was calculated in cells treated with the mixed drugs. c A549 cells and transfected cells were treated with the same concentrations of mixed drugs for 24 h, followed by Western blot for the detection of Ku80 and cleaved caspase-3 protein levels. d Quantification of Ku80 and cleaved caspase-3 levels as shown in (c). Data represented mean ± SD. Each experiment was performed in duplicate or triplicate. +: drug-treated groups; -: non-treated groups. *p < 0.05

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