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Fig. 5 | Respiratory Research

Fig. 5

From: Ku80 correlates with neoadjuvant chemotherapy resistance in human lung adenocarcinoma, but reduces cisplatin/pemetrexed-induced apoptosis in A549 cells

Fig. 5

A549 cell transfection and cisplatin/pemetrexed treatment. a Normal A549 cell lines transfected by lentiviral vector. A549kd = A549 with Ku80-silencing cells. NCkd = A549 cells transfected by nonsilencing shRNA control vector. A549oe = A549 with Ku80-oversxpression cells. NCoe = A549 cells transfected by empty vector for over-expression. b The decreased and increased expression level of Ku80 in A549 cells transfected by Ku80 shRNA and cDNA, respectively. c Relative protein level of Ku80 as shown in (b). d A549 cells and transfected cells were treated with mixture of cisplatin and pemetrexed at concentration of 0, 0.125, 0.25, 0.5, 1, 2, 4, 8 μM for 24 h. Cell viability was performed using the CCK8 assay. Each experiment was performed in duplicate or triplicate. Data were shown as the mean ± SD. *p < 0.05

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