Fig. 5

COPD alveolar macrophage functional polarization in NTHi infection: Alveolar macrophages from COPD patients (n = 6) were infected with NTHi at MOI of 10:1 or 100:1, cells were lysed at 2, 6 and 24 h of exposure, RNA was extracted and gene expression of TNF-α (a), CXCL8 (b), CD38 (c), HLA-DR (d), IL-10 (e), CD36 (f), Mannose receptor (CD206) (g), CD14 (h) and CD163 (i) was assessed by RT-PCR. Data presented as median with range. *, ** represent significant difference of from time-matched basal control; (p < 0.05, <0.01 respectively, Repeated measures ANOVA or Friedman test). Relative expression levels were determined using the ΔΔCt method normalizing to the house keeping gene (GAPDH)