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Fig. 3 | Respiratory Research

Fig. 3

From: Mechanisms of corticosteroid insensitivity in COPD alveolar macrophages exposed to NTHi

Fig. 3

Effect of NTHi on dexamethasone induced nuclear localisation of glucocorticoid receptor. Alveolar macrophages were left untreated (a) or treated with dexamethasone (1 μM) (b), dexamethasone (1 μM) and NTHi (10:1 MOI) (c) or pre-treated with BIRB-796 (1 μM) followed by dexamethasone (1 μM) and NTHi (10:1 MOI) (d). Cells were fixed and immunostained for glucocorticoid receptors (green) and counterstained with 4', 6-diamidino-2-phenylindole nuclear stain (blue). Cells were imaged using fluorescent microscope (X20). Yellow arrows show cells with both cytoplasmic and nuclear localisation of GR, white arrows show cells with nuclear only localisation of GR. Cells expressing nuclear only GR are expressed as percentage of total cells (e). Data represents 4 individual patients with median. Representative images shown for a-d. *represents significantly above basal (p < 0.05). # represents significantly below dexamethasone alone (p < 0.05, Repeated measures two ANOVA with Bonferoni post-test)

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