Fig. 1
From: Mechanisms of corticosteroid insensitivity in COPD alveolar macrophages exposed to NTHi
Effect of dexamethasone on NTHi induced TNF-α, IL-6, CXCL8 and IL-10 in alveolar macrophages from COPD and smokers. Alveolar macrophages from COPD (a, c, e and g) and smokers (b, d, f and h) were pre-treated with dexamethasone (0.0001–1 μM) or with vehicle (DMSO 0.05%) for 1 h before exposure to NTHi either at 10:1 MOI (12 COPD and 8 smokers) or at 100:1 MOI (13 COPD and 10 smokers). Supernatants were collected after 24 h and assayed for TNF-α (a and b), IL-6 (c and d), CXCL8 (e and f) and IL-10 (g and h) release by ELISA. Data presented as Mean ± SEM absolute mediator levels with mean percentage inhibition stated above the bar. *, **, *** represent significant inhibition bellow DMSO control (p < 0.05, 0.01, 0.001 respectively, Repeated measures ANOVA)