Skip to main content
Fig. 3 | Respiratory Research

Fig. 3

From: ABCB1 and ABCG2 drug transporters are differentially expressed in non-small cell lung cancers (NSCLC) and expression is modified by cisplatin treatment via altered Wnt signaling

Fig. 3

Effects of cisplatin treatment. Relative mRNA expression of a) Wnt5a and Wnt7b in cisplatin treated 3D HMVEC-L-NHLF-SAEC co-culture aggregates. mRNA expression of treated samples is compared to untreated controls, n = 3 b) ABCB1 and ABCG2 in cisplatin treated (29.7 μM, 3 h) 3D HMVEC-L-NHLF-SAEC co-culture aggregates. mRNA expression is normalized to beta-actin, n = 3 c) Representative images of Ser675 phosphorylated beta-catenin immune-fluorescent staining in control and cisplatin treated 3D HMVEC-L-NHLF-SAEC aggregates. Fluorescence intensity are representations of three different experiments as mean ± SEM. Scale bar 50 μm, magnification 63×. d) Western-blot analysis and densitometric quantification of Ser675 phosphorylated beta-catenin and beta-catenin proteins in different cell fractions of control and cisplatin treated A549 lung adenocarcinoma cell cultures, n = 3 e) functional activity of ABCB1 and ABCG2 drug transporters in 3D co-culture aggregates following canonical Wnt pathway inhibitor, IWR and inducer, LiCl treatment. Data are presented as mean ± SEM of multidrug resistance activity factor values (MAF), n = 3. MAF values ≤ 20 are considered as MDR negative

Back to article page

Respiratory Research

ISSN: 1465-993X

Contact us