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Fig. 3 | Respiratory Research

Fig. 3

From: Cigarette smoke challenges bone marrow mesenchymal stem cell capacities in guinea pig

Fig. 3

Identification and counting of tracked cells in lung sections under fluorescence microscope. a Lung tissue sections from sham or CS exposed animals 24 h after BS-MSC administration via intratracheal or intravascular route. Tissues were stained with DAPI (blue) for localization of nuclei. BM-MSCs were labelled with PKH67 (green) for cell tracking purposes. Identification and counting of tracked cells in the lung sections was assessed under a fluorescence microscope. Mean values from 20 photographed microscopic fields per animal were calculated. b Box plots of infiltrated BM-MSC in total lung and in the alveolar septa, P value was assessed by Mann–Whitney U-test

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