Table 2 Primer sequences
From: Low tissue levels of miR-125b predict malignancy in solitary fibrous tumors of the pleura
Primer | miRNA | Sequence |
|---|---|---|
Reverse | Universal rev | 5’ - GAG GTA TTC GCA CTG GAT AC - 3’ |
RT | RNU-48 | 5’ - GTC GTA TCC AGT GCA GGG TCC GAG GTA TTC GCA CTG GAT ACG ACG GTC AG - 3’ |
Forward | RNU-48 | 5’ - CCA TGA GTG TGT CGC TGA TG - 3’ |
RT | RNU-49 | 5’ - GTC GTA TCC AGT GCA GGG TCC GAG GTA TTC GCA CTG GAT ACG ACA ATC AG - 3’ |
Forward | RNU-49 | 5’ - AAG CGA TAA CTG ACG AAG ACT AC - 3’ |
RT | let-7a | 5’ - GTC GTA TCC AGT GCA GGG TCC GAG GTA TTC GCA CTG GAT ACG ACA ACT AT - 3’ |
Forward | let-7a | 5’- CGG TGA GGT AGT AGG TTG TAT - 3’ |
RT | miR-16b | 5’ - GTC GTA TCC AGT GCA GGG TCC GAG GTA TTC GCA CTG GAT ACG ACC GCC AA - 3’ |
Forward | miR-16b | 5’- CCT AGC AGC ACG TAA ATA TTG G - 3’ |
RT | miR-17 | 5’ - GTC GTA TCC AGT GCA GGG TCC GAG GTA TTC GCA CTG GAT ACG ACC TAC CT - 3’ |
Forward | miR-17 | 5’ - GCG GCA AAG TGC TTA CAG TG - 3’ |
RT | miR-21 | 5’ - GTC GTA TCC AGT GCA GGG TCC GAG GTA TTC GCA CTG GAT ACG ACT CAA CA - 3’ |
Forward | miR-21 | 5’ - GCC CGC TAG CTT ATC AGA CTG ATG - 3’ |
RT | miR-31 | 5’ - GTC GTA TCC AGT GCA GGG TCC GAG GTA TTC GCA CTG GAT ACG ACA GCT AT - 3’ |
Forward | miR-31 | 5’- CAG GCA AGA TGC TGG CAT AG - 3’ |
RT | miR-34a | 5’ - GTC GTA TCC AGT GCA GGG TCC GAG GTA TTC GCA CTG GAT ACG ACA CAA CC - 3’ |
Forward | miR-34a | 5’ - TGG CAG TGT CTT AGC TGG TTG – 3’ |
RT | miR-92a | 5’ - GTC GTA TCC AGT GCA GGG TCC GAG GTA TTC GCA CTG GAT ACG ACA CAG GC - 3’ |
Forward | miR-92a | 5’ - CCC TAT TGC ACT TGT CCC G - 3’ |
RT | miR-125a | 5’ - GTC GTA TCC AGT GCA GGG TCC GAG GTA TTC GCA CTG GAT ACG ACT CAC AG - 3’ |
Forward | miR-125a | 5’ - TCC CTG AGA CCC TTT AAC C - 3’ |
RT | miR-125b | 5’ - GTC GTA TCC AGT GCA GGG TCC GAG GTA TTC GCA CTG GAT ACG ACT CAC AA - 3’ |
Forward | miR-125b | 5’- CTC CCT GAG ACC CTA ACT TG - 3’ |
RT | miR-195-5b | 5’ - GTC GTA TCC AGT GCA GGG TCC GAG GTA TTC GCA CTG GAT ACG ACG CCA AT - 3’ |
Forward | miR-195-5b | 5’- GGT AGC AGC ACA GAA ATA TTG G - 3’ |
RT | miR-203a | 5’ - GTC GTA TCC AGT GCA GGG TCC GAG GTA TTC GCA CTG GAT ACG ACC TAG TG - 3’ |
Forward | miR-203a | 5’- CCG TGA AAT GTT TAG GAC CAC - 3’ |
RT | miR-223 | 5’ - GTC GTA TCC AGT GCA GGG TCC GAG GTA TTC GCA CTG GAT ACG ACT GGG GT - 3’ |
Forward | miR-223 | 5’- CCC TGT CAG TTT GTC AAA TAC C- 3’ |