Table 2 Primer sequences for quantitative qPCR
Gene | Tm (°C) | Forward Sequence / Reverse sequence | GeneBank accession no. or reference |
|---|---|---|---|
ATP5B | 63° | TCACCCAGGCTGGTTCAGA | NM_001686 |
AGTGGCCAGGGTAGGCTGAT | |||
RPL13A | 63° | AAGGTGGTGGTCGTACGCTGTG | NM_012423 |
CGGGAAGGGTTGGTGTTCATCC | |||
MUC5AC | 65° | CCTTCGACGGACAGAGCTAC | [42] |
TCTCGGTGACAACACGAAAG | |||
FOXJ1 | 65° | GGAGGGGACGTAAATCCCTA | [29] |
TTGGTCCCAGTAGTTCCAGC | |||
HMOX1 | 63° | AACCCTGAACAACGTAGTCTGCGA | NM_002133 |
ATGGTCAACAGCGTGGACACAAA | |||
HSPA5/BiP | 62° | CGAGGAGGAGGACAAGAAGG | NM_001025433 |
CACCTTGAACGGCAAGAACT | |||
DDIT3/CHOP | 62° | GCACCTCCCAGAGCCCTCACTCTCC | NM_001195053.1 |
GTCTACTCCAAGCCTTCCCCCTGCG | |||
PPP1R15A/GADD34 | 62° | ATGTATGGTGAGCGAGAGGC | [43] |
GCAGTGTCCTTATCAGAAGGC | |||
splXBP1 | 62° | TGCTGAGTCCGCAGCAGGTG | [44] |
GCTGGCAGGCTCTGGGGAAG | |||
CXCL8 | 59° | CTG GAC CCC AAG GAA AAC | NM_000584 |
TGG CAA CCC TAC AAC AGA C | |||
DEFB4A/hBD2 | 62° | ATCAGCCATGAGGGTCTTG | NM_004942 |
GCAGCATTTTGTTCCAGG | |||
S100A7 | 60° | ACGTGATGACAAGATTGACAAGC | NM_002963.3 |
GCGAGGTAATTTGTGCCCTTT |