Skip to main content
Fig. 3 | Respiratory Research

Fig. 3

From: Diesel exhaust alters the response of cultured primary bronchial epithelial cells from patients with chronic obstructive pulmonary disease (COPD) to non-typeable Haemophilus influenzae

Fig. 3

Expression of CXCL8 and antimicrobial proteins. ALI-PBECs from COPD and control donors were exposed for 1 h to air (Air) or DE, followed by 3 h exposure to UV-inactivated NTHi (Air + NTHi or DE + NTHi); cultures not exposed in the exposure units but treated with NTHi alone (NTHi) are reported as positive controls. Analysis of gene expression of the neutrophil attracting chemokine CXCL8 a, the antimicrobial peptide DEFB4A b, and S100A7 c is shown. Data are shown as boxes with median, with the whisker indicating the minimum and the maximum values detected. mRNA induction is expressed as fold from the untreated control (indicated by a dashed line) after normalization on two reference genes. Statistical significance of differences is indicated as * p < 0.05, ** p < 0.01 and ***p < 0.001 and was assessed using a two-tailed One-Way ANOVA with Bonferroni’s correction. When combining the non-COPD and COPD groups, a statistically significant induction of CXCL8 mRNA by DE was observed (p = 0.0064; data not shown in the figure) as well as a significant DE-mediated inhibition of the NTHi-induced S100A7 mRNA (p = 0.0155; data not shown in the figure)

Back to article page

Respiratory Research

ISSN: 1465-993X

Contact us