Skip to main content
Fig. 2 | Respiratory Research

Fig. 2

From: Diesel exhaust alters the response of cultured primary bronchial epithelial cells from patients with chronic obstructive pulmonary disease (COPD) to non-typeable Haemophilus influenzae

Fig. 2

Gene expression of heme oxygenase-1 and of genes involved in the integrated stress response (ISR). ALI-PBECs from COPD and control donors were exposed for 1 h to air (Air) or DE, followed by 3 h exposure to UV-inactivated NTHi (Air + NTHi or DE + NTHi). TGFβ (20 ng/ml) is shown as positive control for the oxidative stress response a, tunicamycin (20 ng/ml, Tm) for the BiP expression b and controls with NTHi alone (NTHi). Expression was assessed of HMOX1 mRNA as marker of the oxidative stress response a, and of HSPA5 b, DDIT3/CHOP c and PPP1R15A/GADD34 d as markers of the unfolded protein response (UPR) to ER stress. Data are shown as boxes with median, with the whisker indicating the minimum and the maximum values detected. mRNA induction is expressed as fold from untreated control (indicated by a dashed line) after normalization on two reference genes. Statistical significance of differences is indicated as * p < 0.05, ** p < 0.01 and *** p < 0.001 and was assessed using a two-tailed One-Way ANOVA with Bonferroni’s correction

Back to article page

Respiratory Research

ISSN: 1465-993X

Contact us