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Fig. 1 | Respiratory Research

Fig. 1

From: Diesel exhaust alters the response of cultured primary bronchial epithelial cells from patients with chronic obstructive pulmonary disease (COPD) to non-typeable Haemophilus influenzae

Fig. 1

Epithelial barrier and cytotoxicity. Cells from 5 non-COPD and 7 COPD donors were exposed for 1 h to air (Air) or diesel exhaust (DE) and then incubated for 3 h with or without UV-inactivated NTHi that was added to the apical side (Air + NTHi or DE + NTHi). As controls, inserts not placed in the exposure units were also incubated for 3 h as untreated (U) or with NTHi alone (NTHi). TEER values (trans-electrical epithelial resistance) were measured and expressed as Ohm*cm2 a, while LDH release in the apical and basal compartment was quantified and expressed as % of the positive control (0.01% TRITON-X 100) b. Data are shown as boxes with median, with the whisker indicating the minimum and the maximum values detected. No significant differences were observed (two-tailed One-Way ANOVA with Bonferroni’s correction)

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