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Fig. 3 | Respiratory Research

Fig. 3

From: Effect of a retinoid X receptor partial agonist on airway inflammation and hyperresponsiveness in a murine model of asthma

Fig. 3

IL-4, IL-5, and IL-13 levels in culture supernatants from lung and spleen MNCs. a Experimental protocol. Lung mononuclear cells (MNCs) were isolated from OVA-sensitized and -challenged mice and were cultured for 24 h in the presence or absence of OVA (10 μg/ml) as previously described in the Methods [22]. b (a) IL-4, (b) IL-5, and (c) IL-13 levels in supernatants from cultured lung MNCs isolated from OVA-sensitized and -challenged mice in the presence or absence of OVA (10 μg/ml) were determined by ELISA. c Experimental protocol. Spleen MNCs were isolated from OVA-sensitized mice and were stimulated with OVA for 48 h in the presence or absence of NEt-4IB (1, 10 μM) or prednisone (1 μM) as previously described in the Methods [25]. d (a) IL-4, (b) IL-5, and (c) IL-13 levels in supernatants from cultured spleen MNCs isolated from OVA-sensitized mice in the presence or absence of OVA (10 μg/ml) and NEt-4IB (1, 10 μM) or PSL (1 μM) were determined by ELISA. n = 8–12 in each group. The results for each group are expressed as the mean ± SEM. * Significant differences (P < 0.05) between medium-stimulated OVA/OVA/Vehicle and OVA-stimulated OVA/OVA/Vehicle. # Significant differences between OVA-stimulated OVA/OVA/Vehicle and OVA/OVA/NEt-4IB or OVA/OVA/PSL

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