Fig. 4

Validation of the array data obtained from FFPE PSCC and ISCC samples. a The bar plot compares the fold changes of mRNA levels for four individual genes in ISCC tumours and paired controls obtained either by Human Exon arrays or RT-PCR. b Anti-PTTG1 staining in FFPE ISCC (Tumour) and paired bronchial mucosa (Normal). In ISCC anti-PTTG1 appears moderately in the cytoplasm and strongly in about ~70% of nuclei. The used ISCC is unrelated to the samples for the gene expression study. c H-scores of anti-PTTG1 immunostaining in a set of 45 independent ISCC (Tumour) and paired bronchial epithelia (Normal). Cytoplasmic and nuclear PTTG1 levels are significantly raised in ISCC and therefore cross-validate the gene expression data. Nuclear staining is increased 4x in ISCC whereas the cytoplasmic was raised 1.5x