Fig. 8
From: Pulmonary hypertension and vascular remodeling in mice exposed to crystalline silica
Influx of inflammatory cells in the lung of mice treated with silica and bleomycin. a, Lung sections (5 μm thick) were stained with antibodies specific for neutrophil-specific marker LY-6B (red), macrophage-specific marker CD107b (red) and α-smooth muscle actin (green). Nuclei were stained using DAPI (blue). Images were taken using 20× objective. Representative images of mouse lung from control (images a and d), 0.4 g/kg silica treated (images b and e) or bleomycin treated (images c and f) mice. Increased accumulation of inflammatory cells around vessel (images b and e) found in silica treated lungs. Bar = 200 μm. b, Quantitative analysis of area stained with corresponding specific antibodies. *p < 0.05 when compared to control lung, One Way ANOVA with Holm-Sidak post test