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Fig. 3 | Respiratory Research

Fig. 3

From: Pleural inhibition of the caspase-1/IL-1β pathway diminishes profibrotic lung toxicity of bleomycin

Fig. 3

Pleural delivery of IL-1ra hampers bleomycin-induced TGF-β1 upregulation. a TGF-β1 accumulation in the PLF of NaCl- or BLM-injected mice at D14 was measured by ELISA. Results are expressed as mean ± SEM. n = 8 NaCl, n = 10 BLM. b Representative pictures from TGF-β1 mRNA expression analysis at D14 by FISH in lung sections from mice receiving NaCl (n = 4) or BLM (n = 6). TGF-β1 mRNA: red, DAPI: blue, scale bars: 50 μm. Pleura (dotted line) and TGF-β1 mRNA expression spots (arrows) are indicated. c TGF-β1 protein level assessed by western blot on whole lung from mice co-injected intravenously with NaCl or BLM together with intrapleural IL-1ra (from day 0 to 14) at the indicated doses or NaCl as a control at D21. HSC70 serves as a loading control, n = 3 mice/group. d qPCR analysis for the expression of TGF-β1 mRNA at D21. Expression relative to L32 and normalized with the NaCl group is shown, n = 5 mice/group. e FISH staining for TGF-β1 mRNA expression on lung sections from the above described mice co-injected with BLM and IL-1ra (n = 5 mice/group, scale bars: 50 μm). *p ≤ 0.05, **p ≤ 0.01

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