Skip to main content
Fig. 3 | Respiratory Research

Fig. 3

From: Pulmonary endothelial activation caused by extracellular histones contributes to neutrophil activation in acute respiratory distress syndrome

Fig. 3

Effect of histone-induced endothelial activation on neutrophil activation. Neutrophils were challenged by CTH (40 mg/L, 1 h), then exposed to unchallenged MLVECs. The anti-H4 antibody or non-specific IgG was given concurrently with CTH. The relative percentage of neutrophil adhesion to MLVECs was determined by a cell surface adhesion assay under static conditions (a) and the MPO activity in the supernatant was measured by ELISA (b). MLVECs were challenged by CTH (40 mg/L, 1 h), then exposed to unchallenged Neutrophils. The relative percentage of neutrophil adhesion to MLVECs (c) and the MPO activity in the supernatant (d) were measured. Both MLVECs and neutrophils were challenged by CTH (40 mg/L, 1 h), then exposed to each other. The relative percentage of neutrophil adhesion to MLVECs (e) and the MPO activity in the supernatant (f) were measured. Data are presented as mean ± SD (n = 6). *P < 0.05 vs. the control group, **P < 0.01 vs. the control group; # p < 0.05 vs. the injury group, ## p < 0.01 vs. the injury group

Back to article page