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Fig. 2 | Respiratory Research

Fig. 2

From: Pulmonary endothelial activation caused by extracellular histones contributes to neutrophil activation in acute respiratory distress syndrome

Fig. 2

Effect of extracellular histones on endothelial activation in vitro. The MLVECs were challenged with LPS and then histone H4 in supernatant was measured by Western blot (a). After 10 min of incubation the binding of extracellular histones to the unchallenged endothelial cell membrane was examined by confocal laser microscopy (b). The MLVECs were treated with extracellular histones (1 h) and then P-selectin on endothelium was quantified by cell surface ELISA (c). The vWF in the supernatant was measured by ELISA (d). MLVECs were exposed to CTH and treated concurrently with anti-TLR2 or anti-TLR4 antibody. The inhibitory effect on endothelial activation was measured by P-selectin translocation (e) and release of vWF (f). Data are presented as mean ± SD (n = 6). The results of Western blot and confocal laser microscopy are representative of three similar experiments. *p < 0.05 vs. the control group, ** p < 0.01 vs. the control group; # p < 0.05 vs. the histones (40 mg/L) group, ## p < 0.01 vs. the histones (40 mg/L) group

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