Fig. 1

Role of extracellular histones in endothelial and neutrophil activation in mice with ARDS. Mice were challenged with intravenous LPS (10 mg/kg, 24 h) or CTH (40 mg/kg, 6 h). Anti-H4 antibody (20 mg/kg) or non-specific mouse IgG (20 mg/kg) was injected intravenously once 30 min prior to LPS injection. The levels of circulating vWF and sTM were measured by ELISA (a, b). The translocation of P-selectin was measured by immunohistochemical detection (c, d). Neutrophil infiltration in the lungs was confirmed by immunohistochemical analysis of the specific marker Ly6G and neutrophil activation was examined by MPO activity (e, f). Data are presented as mean ± SD (n = 6). The immunohistochemical results are representative of three similar experiments. *p < 0.05 vs. the control group, ** p < 0.01 vs. the control group; ^# p < 0.05 vs. the LPS group, ^## p < 0.01 vs. the LPS group